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The test uses cell-free circulating fetal DNA (cffDNA) in the maternal serum
§ cffDNA originates from placental trophoblasts (Alberry M et al. 2007)
§ approximately 200 bp in length; smaller than maternal DNA
§ 5 to 7 weeks gestation- increases with gestation age (Wang et al 2013)
§ 11% to 13.4% - amount varies widely among pregnant ladies
§ diminishes rapidly after childbirth (after 2 hours-no longer detectable)
§ Screen for the more common fetal aneuploidies:
§ Trisomy 21 (Down syndrome),
§ Trisomy 18 (Edward syndrome),
§ Trisomy 13 (Patau syndrome) and
§ Monosomy X (Turner syndrome)
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